Nishida J, Ekataksin W, McDonnell D, Urbaschek R, Urbaschek B, McCuskey R S
Department of Anatomy, College of Medicine, University of Arizona, Tucson 85724, USA.
Shock. 1994 Jun;1(6):413-8. doi: 10.1097/00024382-199406000-00004.
The effect of acute ethanol administration on the hepatic microvascular responses to sepsis was studied. Polymicrobial sepsis was induced 30 min after mice had received ethanol (1 g/kg b.w.) or isocaloric maltose-dextrin by gastric gavage. Lethality within 24 h was 91.7% in the ethanol-treated animals and 40.0% in septic controls. Endotoxin levels in ethanol treated animals were 107 pg/ml at 6 hr and 1205 pg/ml at 12 h, compared with 32 pg/ml and 104 pg/ml, respectively in the controls. In vivo microscopy revealed that at 3 h in the ethanol treated septic animals, Kupffer cell phagocytic activity was increased by 41%, whereas the number of sinusoids containing blood flow were reduced by 34% concomitant with a 144% increase in the adherence of leukocytes to the sinusoidal walls when compared with the septic controls. By 6 h, however, Kupffer cell phagocytic activity was reduced by 48% in the ethanol treated animals; this was accompanied by a further deterioration in sinusoidal blood flow. Thus, a small, acute dose of ethanol causes significant impairment of the hepatic microcirculation followed by suppression of Kupffer cell activity. This results in exacerbation of endotoxemia and lethality during polymicrobial sepsis.
研究了急性给予乙醇对肝脏微血管对脓毒症反应的影响。通过胃管给小鼠灌胃乙醇（1 g/kg体重）或等热量的麦芽糖糊精30分钟后，诱导多微生物脓毒症。乙醇处理组动物24小时内的致死率为91.7%，脓毒症对照组为40.0%。乙醇处理组动物6小时时内毒素水平为107 pg/ml，12小时时为1205 pg/ml，而对照组分别为32 pg/ml和104 pg/ml。体内显微镜检查显示，在乙醇处理的脓毒症动物中，3小时时库普弗细胞吞噬活性增加了41%，而与脓毒症对照组相比，有血流的肝血窦数量减少了34%，同时白细胞与肝血窦壁的黏附增加了144%。然而，到6小时时，乙醇处理组动物的库普弗细胞吞噬活性降低了48%；这伴随着肝血窦血流的进一步恶化。因此，小剂量急性乙醇会导致肝脏微循环显著受损，随后库普弗细胞活性受到抑制。这会导致多微生物脓毒症期间内毒素血症和致死率加剧。
