Barberis A, Pearlberg J, Simkovich N, Farrell S, Reinagel P, Bamdad C, Sigal G, Ptashne M
Department of Molecular and Cellular Biology, Harvard University, Cambridge, Massachusetts 02138, USA.
Cell. 1995 May 5;81(3):359-68. doi: 10.1016/0092-8674(95)90389-5.
In yeast strains bearing the point mutation called GAL11P (for potentiator), certain GAL4 derivatives lacking any classical activating region work as strong activators. The P mutation confers upon GAL11, a component of the RNA polymerase II holoenzyme, the ability to interact with a portion of the dimerization region of GAL4. The region of GAL11 affected by the P mutation is evidently functionally inert in ordinary cells, suggesting that this mutation is of no functional significance beyond creating an artificial target for the GAL4 dimerization fragment. From these observations and further analyses of GAL11, we propose that a single activator-holoenzyme contact can trigger gene activation simply by recruiting the latter to DNA.
在带有称为GAL11P（增强子）的点突变的酵母菌株中，某些缺乏任何经典激活区域的GAL4衍生物可作为强激活剂起作用。P突变赋予RNA聚合酶II全酶的一个组分GAL11与GAL4二聚化区域的一部分相互作用的能力。受P突变影响的GAL11区域在普通细胞中显然是功能惰性的，这表明该突变除了为GAL4二聚化片段创造一个人工靶点之外没有功能意义。基于这些观察结果以及对GAL11的进一步分析，我们提出单个激活剂 - 全酶接触可以通过将后者招募到DNA上来触发基因激活。
