Hawthorn L A, Kapanadse B, Yankovsky N, Cowell J K
ICRF Oncology Unit, Institute of Child Health, London, UK.
Cytogenet Cell Genet. 1995;70(1-2):102-3. doi: 10.1159/000134001.
A human chromosome 13-specific cosmid library has been screened with oligonucleotides containing tri- and tetranucleotide repeats with motifs: GACA, GACT, CAC, TCC. 20 cosmid clones were identified and their physical location on chromosome 13 was determined using fluorescence in situ hybridisation. Over 80% of the cosmids were detected using tetranucleotide repeats of which 50% were GACT, but these were distributed along the length of the chromosome. Clones from the 13q12-->q14 and 13q32-->q34 regions were apparently overrepresented and 10% of cosmids were localised to the short arm of the chromosome. These simple tandem repeats will be an important addition to the genetic mapping strategy for human chromosome 13.
已用含有三核苷酸和四核苷酸重复序列且基序为GACA、GACT、CAC、TCC的寡核苷酸筛选了一个人类13号染色体特异性黏粒文库。鉴定出20个黏粒克隆，并使用荧光原位杂交确定了它们在13号染色体上的物理位置。超过80%的黏粒是使用四核苷酸重复序列检测到的，其中50%是GACT，但它们沿染色体长度分布。来自13q12→q14和13q32→q34区域的克隆明显过度富集，10%的黏粒定位于染色体短臂。这些简单串联重复序列将是人类13号染色体遗传图谱策略的重要补充。
