Kost M V, Fedorova L I, Kapanadze B I, Iankovskiĭ N K, Zelenin A V
Mol Biol (Mosk). 1994 Sep-Oct;28(5):1149-57.
Twenty four recombinant cosmids were subregionally localized by fluorescent in situ hybridization on human chromosomes. Fifteen of the clones were found to belong to only one chromosome: 13 clones located on chromosome 13, one located on chromosome 1, and one on chromosome 11. Nine cosmids were located in nuclear organizer regions. The clones gave signals from NOR regions of chromosome 13 and all other chromosomes containing the NOR region. The cosmid probes were selected from the chromosome 13 cosmid library as ones containing microsatellite repeats with motifs GACA, GACT, GATG, TCC, and CA. Each of the 9 clones located in the NOR region contains microsatellites GACA and TCC. Among the 15 clones giving unique signals, we found 9 clones with the GACT microsatellite, and three clones containing one of the microsatellites GATG, TCC, and CA. These microsatellite-containing clones can be used to make polymorphic genetic markers for fine genetic mapping of chromosome 13.
通过荧光原位杂交技术，在人类染色体上对24个重组黏粒进行了亚区域定位。发现其中15个克隆仅属于一条染色体：13个克隆位于13号染色体上，1个位于1号染色体上，1个位于11号染色体上。9个黏粒定位于核仁组织区。这些克隆在13号染色体的核仁组织区以及所有其他含有核仁组织区的染色体上均产生信号。黏粒探针是从13号染色体黏粒文库中挑选出来的，这些探针含有基序为GACA、GACT、GATG、TCC和CA的微卫星重复序列。位于核仁组织区的9个克隆中，每个克隆都含有微卫星GACA和TCC。在给出独特信号的15个克隆中，我们发现9个克隆含有微卫星GACT，3个克隆含有微卫星GATG、TCC和CA中的一种。这些含有微卫星的克隆可用于制作多态性遗传标记，用于13号染色体的精细遗传图谱绘制。
