Tanaka A, Miyamoto K, Matsuo H, Matsumoto K, Yoshida H
Department of Pathology, Faculty of Medicine, Kagoshima University, Japan.
FEBS Lett. 1995 Apr 24;363(3):226-30. doi: 10.1016/0014-5793(95)00324-3.
Androgen-induced growth factor (AIGF) has hormone-regulated properties in the mouse Shionogi carcinoma cell line. To investigate whether or not it is involved in growth of human hormone-responsive cancers, we isolated the human AIGF gene from a placental genomic library. Genomic analyses suggested that the AIGF gene was about 6.5 kilobases in length containing five exons. The deduced amino acid sequence of human AIGF was completely identical with that of the mouse. RT-PCR analyses showed that prostate and breast cancer cell lines, LNCaP, PC-3, and MCF-7, slightly expressed the AIGF gene. Recombinant AIGF enhanced the growth of the human prostate cancer LNCaP cells, and it also markedly stimulated the growth of fibroblasts. These in vitro findings suggest that AIGF might be a possible autocrine or paracrine factor in hormone-responsive cancers.
雄激素诱导生长因子（AIGF）在小鼠紫衫醇癌细胞系中具有激素调节特性。为了研究它是否参与人类激素反应性癌症的生长，我们从胎盘基因组文库中分离出了人类AIGF基因。基因组分析表明，AIGF基因长度约为6.5千碱基，包含五个外显子。人类AIGF推导的氨基酸序列与小鼠的完全相同。逆转录聚合酶链反应（RT-PCR）分析显示，前列腺和乳腺癌细胞系LNCaP、PC-3和MCF-7轻微表达AIGF基因。重组AIGF促进了人类前列腺癌LNCaP细胞的生长，并且还显著刺激了成纤维细胞的生长。这些体外研究结果表明，AIGF可能是激素反应性癌症中一种潜在的自分泌或旁分泌因子。
