Smallwood P M, Munoz-Sanjuan I, Tong P, Macke J P, Hendry S H, Gilbert D J, Copeland N G, Jenkins N A, Nathans J
Department of Molecular Biology and Genetics, Howard Hughes Medical Institute, Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
Proc Natl Acad Sci U S A. 1996 Sep 3;93(18):9850-7. doi: 10.1073/pnas.93.18.9850.
Four new members of the fibroblast growth factor (FGF) family, referred to as fibroblast growth factor homologous factors (FHFs), have been identified by a combination of random cDNA sequencing, data base searches, and degenerate PCR. Pairwise comparisons between the four FHFs show between 58% and 71% amino acid sequence identity, but each FHF shows less than 30% identity when compared with other FGFs. Like FGF-1 (acidic FGF) and FGF-2 (basic FGF), the FHFs lack a classical signal sequence and contain clusters of basic residues that can act as nuclear localization signals. In transiently transfected 293 cells FHF-1 accumulates in the nucleus and is not secreted. Each FHF is expressed in the developing and adult nervous systems, suggesting a role for this branch of the FGF family in nervous system development and function.
通过随机cDNA测序、数据库搜索和简并PCR相结合的方法，已鉴定出成纤维细胞生长因子（FGF）家族的四个新成员，称为成纤维细胞生长因子同源因子（FHFs）。四个FHF之间的两两比较显示氨基酸序列同一性在58%至71%之间，但与其他FGF相比，每个FHF的同一性均低于30%。与FGF-1（酸性FGF）和FGF-2（碱性FGF）一样，FHFs缺乏经典信号序列，且含有可作为核定位信号的碱性残基簇。在瞬时转染的293细胞中，FHF-1积聚在细胞核中且不分泌。每个FHF在发育中的和成年的神经系统中均有表达，提示FGF家族的这一分支在神经系统发育和功能中发挥作用。
