Sklyarova T, Kostyukovski V, Sharov V, Prisyazhnoy V, Denisenko O
Institute of Protein Research, Russian Academy of Sciences, Pushchino, Moscow Region.
FEBS Lett. 1995 Apr 24;363(3):273-6. doi: 10.1016/0014-5793(95)00329-8.
We have studied the effect of actin skeleton depolymerisation induced by C2 toxin on protein synthesis in 3T3 cells. The toxin that was purified from culture medium of Clostridium botulinum type C was shown to specifically ADP-ribosylate actin in vitro and in vivo. Cells exposed to C2 toxin were rounded off, which was accompanied by disappearance of stress fibers. The rate of total protein synthesis decreased two-three times in the treated cells. This correlated with the reduction in amount of polyribosomes. The rates of specific protein synthesis were compared using 2D electrophoresis of pulse-labeled proteins. Dramatic changes were observed in the synthesis of a small group of cellular proteins. Our results indicate that actin filament depolymerization affects gene expression at the level of translation and/or through the control of mRNA concentrations.
我们研究了C2毒素诱导的肌动蛋白骨架解聚对3T3细胞蛋白质合成的影响。从C型肉毒杆菌培养基中纯化的毒素在体外和体内均显示能特异性地将肌动蛋白进行ADP-核糖基化。暴露于C2毒素的细胞变圆，同时应力纤维消失。处理过的细胞中总蛋白质合成速率降低了两到三倍。这与多核糖体数量的减少相关。使用脉冲标记蛋白质的二维电泳比较了特定蛋白质的合成速率。在一小部分细胞蛋白质的合成中观察到了显著变化。我们的结果表明，肌动蛋白丝解聚在翻译水平和/或通过控制mRNA浓度影响基因表达。
