使用接合型和温度敏感型克隆载体将转座子递送至耻垢分枝杆菌。
Use of conjugative and thermosensitive cloning vectors for transposon delivery to Mycobacterium smegmatis.
作者信息
Gavigan J A, Guilhot C, Gicquel B, Martin C
机构信息
Departamento de Microbiología, Facultad de Medicina, Universidad de Zaragoza, Spain.
出版信息
FEMS Microbiol Lett. 1995 Mar 15;127(1-2):35-9. doi: 10.1111/j.1574-6968.1995.tb07446.x.
Conjugative, thermosensitive shuttle plasmids capable of transfer from Escherichia coli to Mycobacterium smegmatis were constructed. They contain both an E. coli replicon and a thermosensitive derivative of the pAL5000 mycobacterial replicon. Using a temperature shift protocol, the conjugative plasmid, pJAZ11 was used to deliver the transposon Tn611 from E. coli into the chromosome of M. smegmatis. Analysis of transconjugants revealed the random insertion of the transposon.
构建了能够从大肠杆菌转移至耻垢分枝杆菌的接合型热敏穿梭质粒。它们同时含有一个大肠杆菌复制子和pAL5000分枝杆菌复制子的热敏衍生物。采用温度转换方案，利用接合型质粒pJAZ11将转座子Tn611从大肠杆菌导入耻垢分枝杆菌的染色体。对接合子的分析显示转座子随机插入。
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