凝固酶阴性葡萄球菌临床重要菌株的流行病学分型

Epidemiological typing of clinically significant strains of coagulase-negative staphylococci.

作者信息

Martin de Nicolás M M, Vindel A, offez-Nieto J A

机构信息

Laboratorio de Referencia de Staphylococcus aureus, Instituto de Salud Carlos III, Madrid, Spain.

出版信息

J Hosp Infect. 1995 Jan;29(1):35-43. doi: 10.1016/0195-6701(95)90291-0.

DOI:10.1016/0195-6701(95)90291-0

PMID:7738338

Abstract

This study was undertaken to determine the characteristics of 202 isolates of coagulase-negative staphylococci (CNS) isolated from 78 patients (two-seven per patient) using biochemical identification, bacteriophage susceptibility patterns, reverse-typing, plasmid profiles, antimicrobial susceptibility testing and slime production. All the strains could be typed by using the six markers consecutively. In addition, we have been able to deduce the similarity of 58 strains from 24 patients by means of one marker or another (these are similar or different strains within one patient but not between one patient and another). The use of a combination of markers is the ideal method for typing the strains; thus making it possible to confirm if two or more isolates from the same patient are similar or not and if they produce infection or are simple contaminants.

摘要

本研究旨在通过生化鉴定、噬菌体敏感性模式、反向分型、质粒图谱、抗菌药物敏感性测试和黏液产生情况，确定从78例患者身上分离出的202株凝固酶阴性葡萄球菌（CNS）（每位患者分离出2 - 7株）的特征。所有菌株均可通过连续使用这六种标记进行分型。此外，我们还能够通过一种或另一种标记推断出24例患者中58株菌株的相似性（这些菌株是同一患者体内的相似或不同菌株，但不是不同患者之间的）。使用多种标记组合是对菌株进行分型的理想方法；从而能够确认同一患者的两个或更多分离株是否相似，以及它们是否引起感染或只是单纯的污染物。