Boehncke W H, Dressel D, Manfras B, Zollner T M, Wettstein A, Böhm B O, Sterry W
Department of Dermatology, University of Ulm, Germany.
J Invest Dermatol. 1995 May;104(5):725-8. doi: 10.1111/1523-1747.ep12606966.
Preferential usage of certain T-cell receptors by the lymphocytic infiltrate in psoriasis might indicate the involvement of an antigen in the pathogenesis of this disease. However, to date there are no data on the complete T-cell-receptor V alpha and V beta repertoire in psoriatic patients. We therefore compared the usage of T-cell-receptor variable regions in blood and skin of 10 patients with chronic plaque-stage psoriasis by means of semiquantitative polymerase chain reaction. Additionally, HLA class II alleles were analyzed by means of sequence-specific oligonucleotide typing. A considerable restriction of the T-cell-receptor repertoire was observed in the skin, where up to 20% of the variable regions present in the blood were not detectable. This was true for both alpha- and beta-chains. However, no interindividually constant pattern of T-cell-receptor restriction was deducible. Inconsistently, a certain preferential usage of some beta chains occurred within the cutaneous compartment. This report on the complete T-cell-receptor V alpha and V beta repertoire in psoriasis documents the restricted receptor repertoire of infiltrating T cells and a lack of enrichment of superantigen-associated V beta regions. Thus superantigens seem not to play a pathogenetically relevant role in chronic plaque-stage psoriasis.
银屑病中淋巴细胞浸润对某些T细胞受体的优先使用可能表明一种抗原参与了该疾病的发病机制。然而，迄今为止，尚无关于银屑病患者完整T细胞受体Vα和Vβ库的数据。因此，我们通过半定量聚合酶链反应比较了10例慢性斑块期银屑病患者血液和皮肤中T细胞受体可变区的使用情况。此外，通过序列特异性寡核苷酸分型分析了HLA II类等位基因。在皮肤中观察到T细胞受体库有相当大的限制，血液中存在的可变区中有高达20%在皮肤中无法检测到。α链和β链均是如此。然而，无法推断出个体间T细胞受体限制的恒定模式。不一致的是，在皮肤区域内某些β链出现了一定程度的优先使用。这份关于银屑病完整T细胞受体Vα和Vβ库的报告证明了浸润性T细胞受体库受限，且缺乏与超抗原相关的Vβ区域富集。因此，超抗原似乎在慢性斑块期银屑病的发病机制中不发挥相关作用。
