Hara H, Narakino H, Kiriyama S, Kasai T
Department of Bioscience and Chemistry, Faculty of Agriculture, Hokkaido University, Sapporo, Japan.
J Nutr. 1995 May;125(5):1143-9. doi: 10.1093/jn/125.5.1143.
We examined differences in pancreatic growth, enzyme content and enzyme concentration between rats fed diets containing normal (2.49 g nitrogen/kg diet) or high (7.46 g nitrogen/kg diet) levels of an amino acid mixture and those in rats fed diets containing normal and high levels of casein for 11 d. Rats fed these diets were injected with a potent cholecystokinin antagonist, MK-329 (2.5 mg/kg body wt.d) or with vehicle only. Pancreatic contents (units in pancreas per 100 g body wt) of protein, trypsinogen and chymotrypsinogen were greater in rats fed a high amino acid diet compared with those in rats fed a normal amino acid diet. Proportionate increases in protein and the serine proteases in pancreata of the high amino acid group relative to those of the normal amino acid group were comparable to those of the high casein group relative to the normal casein group. The pancreatic protease concentrations (units/g pancreas) of rats fed the high casein diet and treated with MK-329 were lower than in rats fed high casein but not treated with MK-329. This difference was not observed in rats fed the high amino acid diet. These results demonstrate that pancreatic growth and proteases are induced by dietary amino acids in rats, and the stimulatory effects of amino acids on exocrine pancreas do not depend on cholecystokinin.
我们研究了分别饲喂含正常水平（2.49克氮/千克日粮）或高水平（7.46克氮/千克日粮）氨基酸混合物的日粮的大鼠与分别饲喂含正常水平和高水平酪蛋白的日粮的大鼠之间，胰腺生长、酶含量和酶浓度的差异，为期11天。给饲喂这些日粮的大鼠注射一种强效胆囊收缩素拮抗剂MK - 329（2.5毫克/千克体重·天）或仅注射赋形剂。与饲喂正常氨基酸日粮的大鼠相比，饲喂高氨基酸日粮的大鼠胰腺中的蛋白质、胰蛋白酶原和糜蛋白酶原含量（每100克体重胰腺中的单位数）更高。高氨基酸组胰腺中蛋白质和丝氨酸蛋白酶相对于正常氨基酸组的成比例增加，与高酪蛋白组相对于正常酪蛋白组的情况相当。饲喂高酪蛋白日粮并接受MK - 329处理的大鼠的胰腺蛋白酶浓度（单位/克胰腺）低于饲喂高酪蛋白但未接受MK - 329处理的大鼠。在饲喂高氨基酸日粮的大鼠中未观察到这种差异。这些结果表明，日粮中的氨基酸可诱导大鼠胰腺生长和蛋白酶生成，并且氨基酸对外分泌胰腺的刺激作用不依赖于胆囊收缩素。
