Krishnadath K K, Tilanus H W, van Blankenstein M, Bosman F T, Mulder A H
Department of Surgery, Erasmus University, Rotterdam, The Netherlands.
J Pathol. 1995 Feb;175(2):175-80. doi: 10.1002/path.1711750204.
Accumulation of p53 protein was determined by immunohistochemistry in archival material of biopsy specimens from 102 patients with Barrett's oesophagus with different grades of dysplasia, in 24 oesophageal adenocarcinomas associated with Barrett's oesophagus, and in 23 cases of metaplastic epithelium adjacent to these carcinomas. Immunostaining for the p53 protein was found in 23/102 (23 per cent) cases of the Barrett's oesophagus biopsies and in 12/23 (52 per cent) cases of Barrett's oesophagus adjacent to adenocarcinoma. Significant correlations were found between the grade of dysplasia and p53 immunoreactivity in both Barrett's biopsies without adenocarcinoma (P < 0.001) and Barrett's oesophagus adjacent to adenocarcinoma (P < 0.05). In the adenocarcinomas, intense nuclear immunohistochemical staining for p53 was diffusely or focally present in 20/24 (83 per cent) of the specimens. In Barrett's oesophagus, p53 is a progression marker with high expression in high-grade dysplasia (89 per cent) and adenocarcinoma (83 per cent).
采用免疫组织化学方法，对102例不同程度发育异常的巴雷特食管活检标本存档材料、24例与巴雷特食管相关的食管腺癌以及23例这些癌旁化生上皮病例进行p53蛋白蓄积情况的检测。在巴雷特食管活检标本中，23/102例（23%）检测到p53蛋白免疫染色阳性；在腺癌旁的巴雷特食管中，12/23例（52%）检测到阳性。在无腺癌的巴雷特食管活检标本（P < 0.001）以及腺癌旁的巴雷特食管（P < 0.05）中，发育异常程度与p53免疫反应性之间均存在显著相关性。在腺癌标本中，20/24例（83%）出现p53蛋白强烈的核免疫组化染色，呈弥漫性或局灶性。在巴雷特食管中 p53是一种进展标志物，在高级别发育异常（89%）和腺癌（83%）中高表达。
