Amenta F, Mancini M, Naves F J, Vega J A, Zaccheo D
Sezione di Anatomia Umana, Istituto di Farmacologia, Università di Camerino, Italy.
Mech Ageing Dev. 1995 Jan 13;77(3):149-57. doi: 10.1016/0047-6374(94)01512-k.
The influence of long term treatment with the dihydropyridine-type Ca2+ antagonist darodipine (PY 108-068) on age-dependent changes in calbindin D-28K immunoreactivity in the cerebellar cortex of male Wistar rats was assessed. In 12-month-old rats used as an adult reference group, specific calbindin D-28K immunoreactivity was found within the cytoplasm of Purkinje neurons and their dendritic processes. The number of Purkinje neurons displaying calbindin D-28K immunoreactivity was decreased in the cerebellar cortex of aged in comparison with adult rats. The pattern of calbindin D-28K immunoreactivity was similar in the cerebellar cortex of 24-month-old rats (aged), although a significant decrease in the intensity of immunoreactivity was noticeable. Treatment of aged rats with darodipine for 6 months increased the percentage of immunoreactive Purkinje neurons and the intensity of calbindin D-28K immunoreactivity in the cytoplasm of Purkinje neurons. Calbindin D-28K is a Ca2+ binding protein probably involved in the modulation of Ca2+ homeostasis. The observation of a positive effect of darodipine treatment on calbindin D-28K immunoreactivity in the cerebellar cortex suggests that manipulation of dihydropyridine-type Ca2+ channels may contribute to counter age-dependent changes of Ca2+ homeostasis.
评估了二氢吡啶类钙离子拮抗剂达罗地平（PY 108 - 068）长期治疗对雄性Wistar大鼠小脑皮质中钙结合蛋白D - 28K免疫反应性随年龄变化的影响。在作为成年参照组的12月龄大鼠中，在浦肯野神经元及其树突状突起的细胞质内发现了特异性钙结合蛋白D - 28K免疫反应性。与成年大鼠相比，老年大鼠小脑皮质中显示钙结合蛋白D - 28K免疫反应性的浦肯野神经元数量减少。24月龄大鼠（老年）的小脑皮质中钙结合蛋白D - 28K免疫反应性模式相似，尽管免疫反应强度有显著降低。用达罗地平治疗老年大鼠6个月增加了免疫反应性浦肯野神经元的百分比以及浦肯野神经元细胞质中钙结合蛋白D - 28K免疫反应性的强度。钙结合蛋白D - 28K是一种可能参与调节钙离子稳态的钙离子结合蛋白。达罗地平治疗对小脑皮质中钙结合蛋白D - 28K免疫反应性有积极作用这一观察结果表明，对二氢吡啶类钙离子通道的调控可能有助于对抗钙离子稳态的年龄依赖性变化。
