Zhou D X, Bisanz-Seyer C, Mache R
Laboratoire de Biologie Moléculaire Végétale, Centre National de la Recherche Scientifique, Unité de Recherche Associée 1178, Université Joseph Fourier, Grenoble, France.
Nucleic Acids Res. 1995 Apr 11;23(7):1165-9. doi: 10.1093/nar/23.7.1165.
A cDNA encoding a specific binding activity for the tissue-specific negative cis-element S1F binding site of spinach rps1 was isolated from a spinach cDNA expression library. This cDNA of 0.7 kb encodes an unusual small peptide of only 70 amino acids, with a basic domain which contains a nuclear localization signal and a putative DNA binding helix. This protein, named S1Fa, is highly conserved between dicotyledonous and monocotyledonous plants and may represent a novel class of DNA binding proteins. The corresponding mRNA is accumulated more in roots and in etiolated seedlings than in green leaves. This expression pattern is correlated with the tissue-specific function of the S1F binding site which represses the rps1 promoter preferentially in roots and in etiolated plants.
从菠菜cDNA表达文库中分离出一种编码与菠菜rps1的组织特异性负顺式元件S1F结合位点具有特异性结合活性的cDNA。这个0.7kb的cDNA编码一个仅含70个氨基酸的特殊小肽，其碱性结构域包含一个核定位信号和一个假定的DNA结合螺旋。这种名为S1Fa的蛋白质在双子叶植物和单子叶植物之间高度保守，可能代表一类新型的DNA结合蛋白。相应的mRNA在根和黄化幼苗中的积累量比在绿叶中更多。这种表达模式与S1F结合位点的组织特异性功能相关，该位点优先在根和黄化植物中抑制rps1启动子。
