Min Wang Ki, Lee Kyu Ho, Song Jong Tae, Seo Hak Soo
Department of Agriculture, Forestry and Bioresources, Research Institute for Agriculture and Life Sciences, and Plant Genomics and Breeding Institute, Seoul National University, Seoul, Korea.
Department of Applied Biosciences, Kyungpook National University, Daegu, Korea.
Plant Signal Behav. 2024 Dec 31;19(1):2439252. doi: 10.1080/15592324.2024.2439252. Epub 2024 Dec 9.
The rice small protein OsS1Fa1, a homolog of spinach S1Fa, plays a significant role in drought tolerance, attributed to its transmembrane domain. In this study, we aim to further elucidate the potential roles of OsS1Fa1 in cold and biotic stresses as an inner nuclear membrane protein. Fluorescence analysis confirmed the localization of OsS1Fa1 to the inner nuclear membrane. Utilizing the bimolecular fluorescence complementation (BiFC) and bacterial infiltration assays with OsS1Fa1 and the inner nuclear membrane protein OsSUN1 (Rice Sad1 and UNC84 (SUN) domain containing 1 (SUN1)), we observed fluorescence detection within the inner nuclear membrane, indicating a direct interaction and colocalization between OsS1Fa1 and OsSUN1. Expression analysis revealed that overexpression of OsS1Fa1 induced the expression of various genes associated with cold and defense responses, including (), (), and (). Our findings collectively indicate that OsS1Fa1 plays crucial roles in both abiotic and biotic stress tolerance as an inner nuclear membrane protein.
水稻小蛋白OsS1Fa1是菠菜S1Fa的同源物，因其跨膜结构域而在耐旱性中发挥重要作用。在本研究中，我们旨在进一步阐明OsS1Fa1作为一种内核膜蛋白在寒冷和生物胁迫中的潜在作用。荧光分析证实了OsS1Fa1定位于内核膜。利用双分子荧光互补（BiFC）和细菌浸润试验，将OsS1Fa1与内核膜蛋白OsSUN1（含水稻Sad1和UNC84（SUN）结构域1（SUN1））进行检测，我们在内核膜内观察到荧光信号，表明OsS1Fa1与OsSUN1之间存在直接相互作用和共定位。表达分析表明，OsS1Fa1的过表达诱导了与寒冷和防御反应相关的各种基因的表达，包括（）、（）和（）。我们的研究结果共同表明，OsS1Fa1作为一种内核膜蛋白，在非生物和生物胁迫耐受性中都起着关键作用。
