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环状寡核苷酸与单链DNA形成稳定的杂交复合物。

Looped oligonucleotides form stable hybrid complexes with a single-stranded DNA.

作者信息

Azhayeva E, Azhayev A, Guzaev A, Hovinen J, Lönnberg H

机构信息

Department of Chemistry, University of Turku, Finland.

出版信息

Nucleic Acids Res. 1995 Apr 11;23(7):1170-6. doi: 10.1093/nar/23.7.1170.

DOI:10.1093/nar/23.7.1170
PMID:7739895
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC306827/
Abstract

Several new branched (1, 2), circular (9) and looped oligonucleotides (14-17) were synthesized. 3'-Deoxypsicothymidine was employed to create the site of branching when required. The circular and looped structures were obtained by oxidative disulfide bond formation between mercaptoalkyl tether groups. All the oligonucleotides prepared contained two T11 sequences, and the branched and looped oligomers an additional alternating CT sequence. The melting experiments revealed that the branched oligonucleotides form relatively weak hybrid (double/triple helix) complexes with the single-stranded oligodeoxyribonucleotide, showing a considerable destabilizing effect produced by the structure at the point of branching. The data obtained with looped oligonucleotides demonstrated considerable stabilization of the hybrid (double/triple helix) complexes with the complement. The data reported may be useful in attempting to design new antisense or antigene oligonucleotides capable of forming selective and stable bimolecular hybrid complexes with nucleic acids.

摘要

合成了几种新的分支（1,2）、环状（9）和环状寡核苷酸（14 - 17）。在需要时，使用3'-脱氧异胸腺嘧啶核苷来创建分支位点。通过巯基烷基连接基团之间形成氧化二硫键获得环状和环状结构。制备的所有寡核苷酸都包含两个T11序列，分支和环状寡聚物还包含一个额外的交替CT序列。熔解实验表明，分支寡核苷酸与单链寡脱氧核糖核苷酸形成相对较弱的杂交（双/三螺旋）复合物，显示出分支点处的结构产生了相当大的去稳定作用。环状寡核苷酸获得的数据表明，与互补物形成的杂交（双/三螺旋）复合物具有相当大的稳定性。所报道的数据可能有助于尝试设计能够与核酸形成选择性和稳定双分子杂交复合物的新型反义或反基因寡核苷酸。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cd0/306827/d7fc3e79e098/nar00007-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cd0/306827/d7fc3e79e098/nar00007-0092-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2cd0/306827/d7fc3e79e098/nar00007-0092-a.jpg

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本文引用的文献

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Current concepts in antisense drug design.反义药物设计的当前概念。
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Nucleic Acids Res. 1993 Jul 25;21(15):3405-11. doi: 10.1093/nar/21.15.3405.
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The polypurine tract, PPT, of HIV as target for antisense and triple-helix-forming oligonucleotides.HIV的多聚嘌呤序列（PPT）作为反义寡核苷酸和三链形成寡核苷酸的作用靶点。
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Circular RNA oligonucleotides. Synthesis, nucleic acid binding properties, and a comparison with circular DNAs.环状RNA寡核苷酸。合成、核酸结合特性及与环状DNA的比较。
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