Hasan H A
Botany Department, Faculty of Science, Assiut University, Egypt.
Acta Microbiol Pol. 1994;43(3-4):327-33.
Twenty species of filamentous fungi were tested for their gibberellin and sterol production, with emphasis on the action of some factors on these products. While all cultures produced both gibberellin and sterol in variable amounts, Aspergillus flavus, A. niger, A. ochraceus, Cladosporium cladosporioides, Gibberella zeae, Penicillium funiculosum, P. italicum and P. rubrum were the best producers. The isolates of A. niger isolated from soil and P. italicum isolated from citrus fruit were selected for further studies. Gibberellin was highly excreted by A. niger in medium fortified with 0.3:0.3 mol nitrogen-phosphorus ratio. On the other hand, sterol was accumulated in P. italicum by vegetable oils more than fruit oils. However, in shaken culture, the organic and olive oils retarded sterol formation with enhanced accumulation of fatty acids.
对20种丝状真菌进行了赤霉素和甾醇产量的测试，重点研究了一些因素对这些产物的作用。虽然所有培养物都能产生不同数量的赤霉素和甾醇，但黄曲霉、黑曲霉、赭曲霉、枝孢枝孢、玉米赤霉、绳状青霉、意大利青霉和红青霉是最佳生产者。从土壤中分离得到的黑曲霉菌株和从柑橘类水果中分离得到的意大利青霉菌株被选作进一步研究对象。在氮磷比为0.3:0.3的强化培养基中，黑曲霉能大量分泌赤霉素。另一方面，植物油比果油更能使意大利青霉积累甾醇。然而，在振荡培养中，有机油和橄榄油会抑制甾醇的形成，同时脂肪酸的积累增加。
