Rumpel E, Michna H, Kühnel W
Institut für Anatomie, Medizinischen Universität zu Lübeck.
Ann Anat. 1995 Mar;177(2):133-8. doi: 10.1016/S0940-9602(11)80060-9.
We used an antibody to the proliferating cell nuclear antigen (PCNA) to investigate the effect of the long-term administration of tamoxifen on proliferative activity in the uterus of mature rats. Untreated cycling and ovariectomized rats served as controls. The PCNA labelling indices (PI) and the mitotic indices (MI) were estimated for the luminal and glandular epithelium and for the stromal fibroblasts. A strong correlation was found for PI and MI in the luminal and in the glandular epithelium, and a lower, but also significant correlation, for the endometrial stroma cells. Tamoxifen treatment decreased the PI of the luminal epithelial cells and of the stroma as much as ovariectomy. In both of these groups, the proportion of anti-PCNA positive cells in the glandular epithelium was significantly higher than in the luminal epithelium. These data indicate that tamoxifen has a strong antiproliferative effect on the uterus of mature rats, and that this antiestrogenic action is cell type specific.
我们使用一种针对增殖细胞核抗原（PCNA）的抗体，来研究长期给予他莫昔芬对成熟大鼠子宫增殖活性的影响。未接受处理的正常发情周期大鼠和去卵巢大鼠作为对照。对腔上皮、腺上皮以及基质成纤维细胞的PCNA标记指数（PI）和有丝分裂指数（MI）进行了评估。在腔上皮和腺上皮中，PI与MI之间存在很强的相关性，而对于子宫内膜基质细胞，相关性较低但也很显著。他莫昔芬处理使腔上皮细胞和基质的PI降低程度与去卵巢相同。在这两组中，腺上皮中抗PCNA阳性细胞的比例显著高于腔上皮。这些数据表明，他莫昔芬对成熟大鼠子宫具有强烈的抗增殖作用，并且这种抗雌激素作用具有细胞类型特异性。
