Mirabolghasemi Ghadire, Kamyab Zahra
Department of Animal Biology, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran.
Int J Fertil Steril. 2017 Apr-Jun;11(1):47-55. doi: 10.22074/ijfs.2016.4794. Epub 2016 Nov 11.
Polycystic ovary syndrome (PCOS) is one of the most common hormonal disorders that can lead to irregular menstrual cycles and hyperandrogenism. Reduced levels of progesterone and increased estrogen in these women can perpetually stimulate the endometrial tissue of the uterus. In this study, we assess the effect of PCOS induction by estradiol valerate (EV) in a rat model.
In this experimental study, adult female Wistar rats that weighed approximately 200 g were divided into control, sham, and experimental groups (n=6 per group). The experimental group received subcutaneous injections of 2 mg EV for induction of PCOS. We confirmed the presence of PCOS in the experimental group rats. Rats from all groups were subsequently killed, after which their uteri were removed and fixed for histological and cytological analyses. The uterine tissue sections were stained with hematoxylin and eosin (H&E) and iron hematoxylin (iron-H). We examined epithelium height, thickness of the uterus wall, and frequency of the mitotic cells. The data were assessed at α=0.05.
Uterine tissue findings from the experimental group showed significant increases in the height of the uterus luminal epithelium, the thickness of the uterus wall, and the frequency of eosinophils in the endometrial stroma. We observed an increased frequency of mitotic cells in the experimental group in both luminal and glandular epithelia of the uterus. An increased rate of the glandular epithelium region was noticeable and significant.
Induction of PCOS by EV could change the proliferation rate in the endo- metrial tissue of the uterus.
多囊卵巢综合征（PCOS）是最常见的激素紊乱疾病之一，可导致月经周期不规律和高雄激素血症。这些女性体内孕酮水平降低和雌激素水平升高会持续刺激子宫内膜组织。在本研究中，我们评估了戊酸雌二醇（EV）诱导大鼠模型多囊卵巢综合征的效果。
在本实验研究中，将体重约200 g的成年雌性Wistar大鼠分为对照组、假手术组和实验组（每组n = 6）。实验组皮下注射2 mg EV以诱导多囊卵巢综合征。我们证实了实验组大鼠存在多囊卵巢综合征。随后处死所有组的大鼠，取出子宫并固定以进行组织学和细胞学分析。子宫组织切片用苏木精和伊红（H&E）以及铁苏木精（iron-H）染色。我们检查了上皮高度、子宫壁厚度和有丝分裂细胞频率。数据以α = 0.05进行评估。
实验组子宫组织结果显示，子宫腔上皮高度、子宫壁厚度以及子宫内膜基质中嗜酸性粒细胞频率显著增加。我们观察到实验组子宫腔和腺上皮中有丝分裂细胞频率增加。腺上皮区域的增加率显著且明显。
EV诱导多囊卵巢综合征可改变子宫内膜组织的增殖率。
