Ofori-Anyinam O, Vrijsen R, Kronenberger P, Boeyé A
Department of Microbiology and Hygiene, Vrije Universiteit Brussel, Belgium.
Antiviral Res. 1995 Jan;26(1):27-35. doi: 10.1016/0166-3542(94)00063-e.
Poliovirus type 1 (Mahoney) was treated with the capsid-binding pyridazinamine R 78206, followed by dialysis to remove free compound. Upon infection of HeLa cells by R 78206-pretreated virus, the formation of intra- and extracellular modified particles was completely inhibited, except for a small amount of empty capsids. The synthesis of viral proteins and first cycle progeny virus was delayed by 1 h. The results suggest that poliovirus infection does not require intracellular accumulation of 135 S eclipse particles.
1型脊髓灰质炎病毒（马奥尼株）用衣壳结合哒嗪胺R 78206处理，随后透析以去除游离化合物。用R 78206预处理的病毒感染HeLa细胞后，除少量空衣壳外，细胞内和细胞外修饰颗粒的形成被完全抑制。病毒蛋白和第一代子代病毒的合成延迟1小时。结果表明，脊髓灰质炎病毒感染不需要135S蚀斑颗粒在细胞内积累。
