Vyth-Dreese F A, Dellemijn T A, van Oostveen J W, Feltkamp C A, Hekman A
Division of Immunology, The Netherlands Cancer Institute, Amsterdam.
Blood. 1995 May 15;85(10):2802-12.
Peripheral blood lymphocytes of a patient with follicular B-cell non-Hodgkin's lymphoma (B-NHL) were immortalized in vitro by Epstein-Barr virus (EBV). Eight cell lines were obtained (termed BNS1, BNS2-1 through BNS2-7), which showed a pattern of idiotypic (id) Ig surface expression and Ig JH and kappa gene rearrangement, identical to that of the parent cells (termed NS), confirming their neoplastic origin. Induction of allogeneic T-cell proliferation by NS cells was mediated by HLA-DR, leukocyte function-associated antigen-1 (LFA-1), LFA-3, B7-1/CD80, and CTLA4 and resulted in the upregulation (LFA-3, intercellular adhesion molecule-1 [ICAM-1], CD40) and induction (B7-1/CD80, B7-2/CD86, L16/activated LFA-1) of accessory molecules on NS cells. In turn, responder T lymphocytes were induced to express B7-1/CD80, B7-2/CD86, CD40 ligand (CD40L), ICAM-1, L16/activated LFA-1, and HLA-DR, reflecting bidirectional signaling between T lymphocytes and B-NHL cells. Preactivation of NS cells by EBV transformation or CD40 engagement resulted in enhanced expression of accessory molecules and abolished the requirement for accessory cells during allostimulation. These resting and activated clonal B cells will be useful in further dissecting the requirements for B-NHL costimulation.
一名滤泡性B细胞非霍奇金淋巴瘤（B-NHL）患者的外周血淋巴细胞通过爱泼斯坦-巴尔病毒（EBV）在体外实现永生化。获得了8个细胞系（称为BNS1、BNS2-1至BNS2-7），它们显示出独特型（id）Ig表面表达模式以及Ig JH和κ基因重排，与亲本细胞（称为NS）相同，证实了它们的肿瘤起源。NS细胞诱导的同种异体T细胞增殖由HLA-DR、白细胞功能相关抗原-1（LFA-1）、LFA-3、B7-1/CD80和CTLA4介导，并导致NS细胞上辅助分子的上调（LFA-3、细胞间黏附分子-1 [ICAM-1]、CD40）和诱导（B7-1/CD80、B7-2/CD86、L16/活化的LFA-1）。反过来，反应性T淋巴细胞被诱导表达B7-1/CD80、B7-2/CD86、CD40配体（CD40L）、ICAM-1、L16/活化的LFA-1和HLA-DR，反映了T淋巴细胞与B-NHL细胞之间的双向信号传导。通过EBV转化或CD40参与对NS细胞进行预激活导致辅助分子表达增强，并消除了同种异体刺激过程中对辅助细胞的需求。这些静息和活化的克隆性B细胞将有助于进一步剖析B-NHL共刺激的需求。
