Kuriyama Y, Nakano M, Kawanishi Y, Iwase O, Kuge S, Toyama K
Department of Internal Medicine, Tokyo Medical College, Japan.
Leuk Lymphoma. 1994 Mar;13(1-2):123-9. doi: 10.3109/10428199409051662.
In an attempt to determine the roles of adhesion molecules in the formation and deterioration of neoplastic follicles, we used flow cytometry to investigate how strongly neoplastic B-cells express VLA-4 alpha and LFA-1 alpha on their surfaces. Neoplastic and normal B-cells were taken from 24 patients with B-cell non-Hodgkin's lymphomas (B-NHL) and 6 with B-cell chronic lymphocytic leukemia (B-CLL). The expression intensities of the adhesion molecules were graded as follows: (-), (+), (+2) and (+3). Normal B-cells expressed those molecules with an intensity of (+2). The data for VLA-4 alpha expression were as follows: follicular B-NHL [10/11; (+2) and 1/11; (+)], partially follicular [5/5; (+)], diffuse [8/8; (+)] and B-CLL [6/6; (-)]. Those for LFA-1 alpha were as follows: follicular B-NHL [7/11; (+2), 4/11; (+)], partially follicular [3/5; (+2), 2/5; (+)], diffuse [3/8; (+2), 5/8; (+)] and B-CLL [3/6; (+), 3/6; (-)]. These results suggest that VLA-4 molecules expressed on neoplastic B-cells may be involved closely in the formation and deterioration of neoplastic follicles, although the expression of LFA-1 molecules seems to play only a minor part in such events.
为了确定黏附分子在肿瘤性滤泡形成和恶化过程中的作用，我们使用流式细胞术来研究肿瘤性B细胞在其表面表达VLA-4α和LFA-1α的强度。肿瘤性B细胞和正常B细胞取自24例B细胞非霍奇金淋巴瘤（B-NHL）患者和6例B细胞慢性淋巴细胞白血病（B-CLL）患者。黏附分子的表达强度分级如下：（-）、（+）、（+2）和（+3）。正常B细胞以（+2）的强度表达这些分子。VLA-4α表达的数据如下：滤泡性B-NHL [10/11；（+2）和1/11；（+）]，部分滤泡性[5/5；（+）]，弥漫性[8/8；（+）]和B-CLL [6/6；（-）]。LFA-1α的数据如下：滤泡性B-NHL [7/11；（+2），4/11；（+）]，部分滤泡性[3/5；（+2），2/5；（+）]，弥漫性[3/8；（+2），5/8；（+）]和B-CLL [3/6；（+），3/6；（-）]。这些结果表明，肿瘤性B细胞上表达的VLA-4分子可能与肿瘤性滤泡的形成和恶化密切相关，尽管LFA-1分子的表达在这些事件中似乎只起次要作用。
