Momosaki S, Yano H, Kojiro M
First Department of Pathology, Kurume University, School of Medicine, Japan.
Pathol Int. 1995 Feb;45(2):137-46. doi: 10.1111/j.1440-1827.1995.tb03434.x.
A human hepatic adenosquamous carcinoma cell line (KMC-2) was established from a serially transplanted tumor in nude mice (nuKMC-2), which originated from human cholangio-cellular carcinoma and showed histological alteration from adenocarcinoma to squamous cell carcinoma (SCC) along with serial transplantation. KMC-2 cells in monolayer culture proliferated in a sheet-like arrangement with a population doubling time of 29.5 h, whereas the cells in 0.1% collagen gel embedded culture formed a compact and tubular structure with the population doubling time of 35.4 h. The cells secreted carbohydrate antigen 19-9 (CA19-9), tissue polypeptide antigen and SCC-related antigen. The back-transplanted nude mouse tumor exhibited morphologic features of adenosquamous carcinoma resembling those in the original nude mouse tumor. IFN-alpha, IFN-gamma and TNF-alpha suppressed cell proliferation significantly. Functionally, IFN-gamma significantly suppressed CA19-9 secretion, and conversely promoted SCC-related antigen secretion. These findings suggest that KMC-2 is the first human hepatic adenosquamous carcinoma cell line primarily originated from adenocarcinoma; the environmental factors, such as the presence of extracellular matrix and the cytokines influenced the growth, morphology and function of KMC-2.
一种人肝腺鳞癌细胞系（KMC - 2）是从裸鼠体内连续传代移植的肿瘤（nuKMC - 2）中建立的，该肿瘤起源于人胆管细胞癌，并且随着连续移植显示出从腺癌到鳞状细胞癌（SCC）的组织学改变。单层培养的KMC - 2细胞呈片状排列增殖，群体倍增时间为29.5小时，而在包埋于0.1%胶原凝胶中的培养物中的细胞形成紧密的管状结构，群体倍增时间为35.4小时。这些细胞分泌糖类抗原19 - 9（CA19 - 9）、组织多肽抗原和SCC相关抗原。回输移植的裸鼠肿瘤表现出腺鳞癌的形态学特征，类似于原始裸鼠肿瘤中的特征。干扰素α、干扰素γ和肿瘤坏死因子α显著抑制细胞增殖。在功能上，干扰素γ显著抑制CA19 - 9分泌，相反促进SCC相关抗原分泌。这些发现表明，KMC - 2是首个主要起源于腺癌的人肝腺鳞癌细胞系；细胞外基质的存在和细胞因子等环境因素影响了KMC - 2的生长、形态和功能。
