Iemura A, Maruiwa M, Yano H, Kojiro M
First Department of Pathology, Kurume University School of Medicine, Japan.
J Hepatol. 1992 Jul;15(3):288-98. doi: 10.1016/0168-8278(92)90058-w.
We have recently established a cholangiocellular carcinoma (CCC) cell line, designated KMC-1, from a nude mouse subcutaneous tumor which developed after inoculation of a surgically resected peripheral type CCC from a 62-year-old Japanese male patient. KMC-1 cells grew over a 26-month period and passaged 57 times. These cells retained the morphologic characteristics of both the original tumor and the subcutaneous tumor in the nude mouse, which mainly consisted of irregular tubules and invaded surrounding interstitial tissue in part with an indurate pattern. KMC-1 cells grew in a monolayer pavement-like cell arrangement with tubular formation in part. Some cells and/or glands had a mucin-like substance inside. The doubling time of KMC-1 cells growing in serum-containing medium was 54 h at passage 31. Cell growth in serum-free medium was slow but steady. The number of chromosomes was distributed in range from 73 to 83 with modes of 76 and 78. KMC-1 cells secreted some tumor markers such as DUPAN-2, CA125, TPA, hCG, CA19-9 and ferritin, however, the secretion of DUPAN-2, and CA19-9 and ferritin were only detectable in serum-containing and serum-free medium, respectively. These findings suggest that KMC-1 cells will provide a variety of experimental models for research on CCC and the mechanisms of tumor marker secretion.
我们最近从一只裸鼠皮下肿瘤中建立了一种胆管细胞癌（CCC）细胞系，命名为KMC-1。该裸鼠皮下肿瘤是在接种一名62岁日本男性患者手术切除的外周型CCC后形成的。KMC-1细胞生长了26个月，传代57次。这些细胞保留了原始肿瘤和裸鼠皮下肿瘤的形态学特征，主要由不规则小管组成，部分呈硬结状侵入周围间质组织。KMC-1细胞呈单层铺路样细胞排列生长，部分形成管状结构。一些细胞和/或腺体内部含有黏蛋白样物质。在含血清培养基中生长的KMC-1细胞在第31代时的倍增时间为54小时。在无血清培养基中细胞生长缓慢但稳定。染色体数目分布在73至83之间，众数为76和78。KMC-1细胞分泌一些肿瘤标志物，如DUPAN-2、CA125、TPA、hCG、CA19-9和铁蛋白，然而，DUPAN-2、CA19-9和铁蛋白的分泌分别仅在含血清和无血清培养基中可检测到。这些发现表明，KMC-1细胞将为CCC研究和肿瘤标志物分泌机制提供多种实验模型。
