Kroese F G, de Boer N K, de Boer T, Nieuwenhuis P, Kantor A B, Deenen G J
Department of Histology and Cell Biology, University of Groningen, The Netherlands.
Cell Immunol. 1995 May;162(2):185-93. doi: 10.1006/cimm.1995.1068.
In rats, the glycoprotein Thy-1 is expressed on recently bone marrow (BM)-generated B cells but not on mature recirculating follicular (RF) B cells. Here we demonstrate that Thy-1+ B cells consist of two phenotypically distinct, but developmentally related, populations: a population of newly formed (NF) B cells (IgMbr-IgDdu) that give rise to the second, less immature, Thy-1+ population of so-called early recirculating follicular (ERF) B cells (Thy-1+IgMduIgDbr) cells. These cells ultimately develop to RF-B cells (Thy-1-IgMbrIgDdu). Kinetic studies reveal that in absolute numbers per day most cells die at the transition of NF-B cells in the BM and those in the periphery: less cells die at later stages of B cell differentiation. Given the notion that this cell loss is not random, we speculate that NF-B cells and ERF-B cells may represent crucial steps during peripheral B cell development and their selection. Identification of their unique phenotype makes it possible to evaluate their roles in development of the antibody repertoire.
在大鼠中，糖蛋白Thy-1在最近由骨髓（BM）产生的B细胞上表达，但在成熟的循环滤泡（RF）B细胞上不表达。在这里，我们证明Thy-1+B细胞由两个表型不同但发育相关的群体组成：一群新形成的（NF）B细胞（IgMbr-IgDdu），它们产生了第二个不太幼稚的Thy-1+群体，即所谓的早期循环滤泡（ERF）B细胞（Thy-1+IgMduIgDbr）。这些细胞最终发育为RF-B细胞（Thy-1-IgMbrIgDdu）。动力学研究表明，每天绝对数量的大多数细胞在骨髓中NF-B细胞向外周NF-B细胞转变时死亡：在B细胞分化后期死亡的细胞较少。鉴于这种细胞损失并非随机发生的观点，我们推测NF-B细胞和ERF-B细胞可能代表外周B细胞发育及其选择过程中的关键步骤。鉴定它们独特的表型使得评估它们在抗体库发育中的作用成为可能。
