Schilling A, Glusa E, Müller-Schweinitzer E
Institute of Pharmacology and Toxicology, Medical Academy, Erfurt, Germany.
Cryobiology. 1995 Apr;32(2):109-13. doi: 10.1006/cryo.1995.1010.
Rings from human saphenous vein and its side branches were randomly distributed into three groups for investigations in vitro unfrozen and after storage for 2-8 weeks at -196 degrees C while suspended in media containing fetal calf serum (FCS) or Krebs-Henseleit (KH) solution as vehicles for the cryoprotecting agents (2.0 M Me2SO and 0.1 M sucrose). After thawing, the contractile responses to agonists such as noradrenaline, 5-hydroxytryptamine, and the thromboxane analogue U46619 were reduced by about 30-40%. Endothelium-independent relaxant responses to papaverine and the potassium channel activator bimakalim were well maintained and similar to untreated control tissues. The use of KH solution instead of FCS as the cryoprotectant vehicle did not negatively influence post-thaw functional recovery, suggesting that for optimal functional preservation of human venous smooth muscle the presence of FCS in the cryomedium is not required.
取自人隐静脉及其侧支的血管环被随机分为三组，分别用于在未冷冻状态下以及在-196℃储存2至8周后进行体外研究，血管环悬浮于含有胎牛血清（FCS）或Krebs-Henseleit（KH）溶液的培养基中，作为冷冻保护剂（2.0M二甲基亚砜和0.1M蔗糖）的载体。解冻后，血管环对去甲肾上腺素、5-羟色胺和血栓素类似物U46619等激动剂的收缩反应降低了约30%-40%。对罂粟碱和钾通道激活剂苄环烷的内皮依赖性舒张反应得到良好维持，且与未处理的对照组织相似。使用KH溶液而非FCS作为冷冻保护剂载体对解冻后的功能恢复没有负面影响，这表明为了使人静脉平滑肌获得最佳功能保存，冷冻介质中不需要有FCS。
