Hang H, Fox M H
Department of Radiological Health Sciences, Colorado State University, Fort Collins 80523, USA.
Cytometry. 1995 Feb 1;19(2):119-25. doi: 10.1002/cyto.990190206.
In response to a heat shock, the synthesis of heat shock proteins with a molecular weight of 72 kD (hsp72) is induced. To determine whether hsp72 expression in Chinese hamster ovary (CHO) cells is cell cycle associated, flow cytometry was used to measure DNA content and heat shock protein levels simultaneously. After 10 min at 45.0 degrees C and incubation at 37 degrees C for several hours, hsp72 was induced to a high level in early S phase. Inhibitors of DNA synthesis, aphidicolin, and 1-beta-D-arabinofuranosylcytosine (araC), suppressed the induction of hsp72 but did not inhibit it completely. Hsp73 (constitutive hsp70) was also induced by heat shock, but the induction was uniform through the cell cycle and it was not suppressed by araC.
作为对热休克的反应，会诱导合成分子量为72千道尔顿的热休克蛋白（hsp72）。为了确定中国仓鼠卵巢（CHO）细胞中hsp72的表达是否与细胞周期相关，采用流式细胞术同时测量DNA含量和热休克蛋白水平。在45.0摄氏度下处理10分钟并在37摄氏度下孵育数小时后，hsp72在S期早期被诱导至高水平。DNA合成抑制剂阿非迪霉素和1-β-D-阿拉伯呋喃糖基胞嘧啶（araC）可抑制hsp72的诱导，但不能完全抑制。热休克也会诱导hsp73（组成型hsp70），但其诱导在整个细胞周期中是均匀的，并且不受araC抑制。
