Marchand V, Tournier J M, Chevillard M, Polette M, Beorchia A, Klossek J M, Puchelle E
INSERM U314, Université de Reims, France.
Eur Respir J. 1995 Jan;8(1):15-21. doi: 10.1183/09031936.95.08010015.
Antileucoprotease (ALP) is generally considered as a specific marker for glandular serous cells, and plays a major role in the defence of the respiratory tract against proteolytic damage. Nevertheless, several studies have identified ALP in bronchial and bronchiolar surface epithelial cells, and also an increased number of ALP-containing cells in bronchiolar tissue during the development of pulmonary diseases. In order to define more clearly whether the surface epithelium might be involved in the defence of the respiratory mucosa, we have investigated the expression of ALP by cells of the nasal surface epithelium. Indirect immunocytochemistry and in situ hybridization for ALP were performed on human nasal polyp sections. The height of the surface epithelium, its morphology, and the degree of local inflammation were assessed in parallel. Surface epithelium morphology was highly heterogeneous. ALP-containing cells were identified, but only in remodelled areas of the surface epithelium (foldings, basal cell and/or mucous cell hyperplasia), with no association to the degree of inflammation. These results demonstrate that the surface epithelial cells of the human adult nasal mucosa can express ALP in remodelled surface epithelium, and may be actively involved in the biochemical defence of the airways.
抗白细胞蛋白酶（ALP）通常被认为是腺浆液细胞的一种特异性标志物，在呼吸道抵御蛋白水解损伤中起主要作用。然而，多项研究已在支气管和细支气管表面上皮细胞中鉴定出ALP，并且在肺部疾病发展过程中细支气管组织中含ALP的细胞数量也有所增加。为了更明确地确定表面上皮是否可能参与呼吸道黏膜的防御，我们研究了鼻表面上皮细胞中ALP的表达。对人鼻息肉切片进行了ALP的间接免疫细胞化学和原位杂交。同时评估了表面上皮的高度、形态以及局部炎症程度。表面上皮形态高度异质。已鉴定出含ALP的细胞，但仅存在于表面上皮的重塑区域（褶皱、基底细胞和/或黏液细胞增生），与炎症程度无关。这些结果表明，成人鼻黏膜的表面上皮细胞可在重塑的表面上皮中表达ALP，并可能积极参与气道的生化防御。
