Coste A, Rateau J G, Bernaudin J F, Peynègre R, Escudier E
Service d'ORL, Hôpitaux Henri Mondor et Intercommunal de Créteil, France.
Acta Otolaryngol. 1996 Sep;116(5):755-61. doi: 10.3109/00016489609137920.
In nasal polyps, constantly associated with chronic inflammation, frequent epithelial morphological changes (squamous metaplasia, secretory hyperplasia) suggest a dysregulation of epithelial cell proliferation. Cell proliferation in nasal respiratory epithelium was therefore evaluated in nasal polyposis. In 20 patients, we compared cell proliferation in mucosa from the inferior turbinate to these in nasal polyps using two methods: Flow cytometry analyzing first the ploidy and the percentage of S-phase cells (propidium iodide DNA labeling), secondly the percentage of Ki-67-labeled cells and the green fluorescent index (fluorescein-conjugated anti-human Ki-67 antigen labeling, and thirdly the percentage of Ki-67-labeled cells being in S-phase. Immunohistochemistry, quantifying the expression of Ki-67 antigen in the epithelium permitting to calculate a Ki-67 index. All cell-populations studied were diploid. Percentages of S-phase cells, Ki-67-labeled cells, Ki-67 labeled cells being in the S-phase and green fluorescence index was significantly higher in nasal polyps than in mucosa Ki-67 index were significantly higher in nasal polyps than in mucosa in the epithelium. Epithelial cell proliferation which is therefore increased in nasal polyp could play an important role in nasal polyposis pathogenesis and its relationships with inflammation can be suggested.
在鼻息肉中，常伴有慢性炎症，频繁的上皮形态学改变（鳞状化生、分泌性增生）提示上皮细胞增殖失调。因此，我们在鼻息肉病中评估了鼻呼吸上皮的细胞增殖情况。我们选取了20例患者，使用两种方法比较了下鼻甲黏膜与鼻息肉中的细胞增殖情况：一是流式细胞术，首先分析倍体和S期细胞百分比（碘化丙啶DNA标记），其次分析Ki-67标记细胞百分比和绿色荧光指数（荧光素偶联抗人Ki-67抗原标记），其三分析处于S期的Ki-67标记细胞百分比；二是免疫组织化学，通过定量上皮中Ki-67抗原的表达来计算Ki-67指数。所有研究的细胞群体均为二倍体。鼻息肉中S期细胞百分比、Ki-67标记细胞百分比、处于S期的Ki-67标记细胞百分比以及绿色荧光指数均显著高于黏膜，上皮中的Ki-67指数也显著高于黏膜。因此，鼻息肉中增加的上皮细胞增殖可能在鼻息肉病发病机制中起重要作用，并且可以推测其与炎症的关系。
