Eroles P, Sentandreu M, Elorza M V, Sentandreu R
Departament de Microbiología, Facultat de Farmacia, Universitat de València, Spain.
FEMS Microbiol Lett. 1995 Apr 15;128(1):95-100. doi: 10.1111/j.1574-6968.1995.tb07506.x.
Immunoscreening of a mycelial expression library with polyclonal antibodies raised against mycelial cell wall resulted in the detection of a cDNA encoding a heat shock protein of Candida albicans. Sequence analysis of a 0.8-kb cDNA subclone, 2M-1, revealed an open reading frame encoding 244 amino acids. Southern blot analysis with this fragment as a probe demonstrated hybridization to C. albicans DNA. Northern analysis showed a substantial increase in 2M RNA expression levels after cells were subjected to heat shock. Western blot analysis with 2M monospecific antibodies recognized a 70-kDa protein which was present in membrane particles and cytosolic fractions.
