Choi J H, Yu B P
University of Texas Health Science Center at San Antonio, Department of Physiology 78284-7756, USA.
Free Radic Biol Med. 1995 Feb;18(2):133-9. doi: 10.1016/0891-5849(94)00106-t.
Dietary restriction was used in this study as a modulator of free radical reactions to examine the effects of age on the physico-biochemical property of synaptosomal membranes. Synaptosomal membranes were isolated from the frontal cortices of 6- and 24-month-old barrier-reared male Fischer 344 rats maintained on either an ad lib (AL) or a 40% diet restricted (DR) feeding schedule. The age-related production of reactive oxygen species (ROS) was only seen in the AL group, and dietary restriction suppressed the amount of the reactive species at both ages. Although membrane fluidity significantly decreased with age in AL fed rats, no change occurred in DR rats. Because age-related increases in cholesterol/phospholipid occurred in both AL and DR groups, fluidity loss may be influenced by factors other than cholesterol. We suggest that lipid peroxidation may be a major factor in the change in fluidity during the aging process.
在本研究中，饮食限制被用作自由基反应的调节剂，以研究年龄对突触体膜物理生化特性的影响。从6个月和24个月大的屏障饲养雄性费希尔344大鼠的额叶皮质中分离突触体膜，这些大鼠分别采用自由采食（AL）或40%饮食限制（DR）的喂养方案。活性氧（ROS）与年龄相关的产生仅在AL组中观察到，饮食限制在两个年龄段均抑制了活性物质的量。虽然在AL喂养的大鼠中，膜流动性随年龄显著降低，但在DR大鼠中没有变化。由于在AL组和DR组中胆固醇/磷脂均随年龄增加，流动性丧失可能受胆固醇以外的因素影响。我们认为脂质过氧化可能是衰老过程中流动性变化的主要因素。
