Sun G, Chen L, Wu M
Hua Xi Yi Ke Da Xue Xue Bao. 1994 Dec;25(4):398-401.
This paper reports a direct PCR method for the detection of Alu DNA in 18 different kinds of human tissue using primers of Alu 9.1 and Alu 9.2 without DNA extraction. The results showed that besides fresh tissues, some formalin fixed paraffin embedded tissues can be correctly analyzed with PCR. Different amounts of PCR products were obtained from different types of tissues. The duration of fixation in formalin is an important factor exerting an influence on the quantity of the high molecular weight DNA and the results of PCR. No obvious influence of the duration of storage of paraffin embedded tissues on the PCR results was observed. This method is quite simple, sensitive and rapid to perform. It can be performed under a condition of avoiding contamination of DNA and DNase as well as the detrimental phenol and chloroform.
本文报道了一种直接PCR方法，该方法使用Alu 9.1和Alu 9.2引物，无需提取DNA，即可检测18种不同类型人体组织中的Alu DNA。结果表明，除新鲜组织外，一些福尔马林固定石蜡包埋组织也可通过PCR进行正确分析。不同类型的组织获得了不同量的PCR产物。福尔马林固定时间是影响高分子量DNA数量和PCR结果的重要因素。未观察到石蜡包埋组织的储存时间对PCR结果有明显影响。该方法操作非常简单、灵敏且快速。它可以在避免DNA和DNase以及有害的苯酚和氯仿污染的条件下进行。
