Akiba T, Ando K, Marumo F
Second Department of Internal Medicine, Tokyo Medical and Dental University, Japan.
Int J Artif Organs. 1994 Nov;17(11):585-90.
An attempt was made to clarify whether the molecular forms of atrial natriuretic peptide (ANP) in the plasma of stable hemodialysis patients differ from those of healthy volunteers, and whether the ANP molecular forms in plasma might change during hemodialysis treatment. Ten stable hemodialysis patients with no clinical signs of cardiac disease were treated for 4 hours by a hollow fiber-type dialyzer. Plasma ANP concentrations before dialysis were 210 +/- 101.6 pg/ml (mean +/- SD), which were significantly higher than that of volunteers (59.2 +/- 37.2 pg/ml, n = 25). They were significantly decreased to 71.6 +/- 60.1 pg/ml after dialysis. Molecular patterns of ANP were measured by gel permeation chromatography and reverse-phase high performance liquid chromatography. Immunoreactive alpha-ANP peaks of GPC, which co-migrated with authentic alpha-, beta-, and gamma-ANP, were supposed to be alpha-, beta-, and gamma-ANP. The plasma of four patients contained a beta-ANP peak before dialysis, and three of the four still contained a beta-ANP peak after dialysis. These results showed that the middle-molecular-weight ANP, which co-migrated with authentic beta-ANP and is supposed to be beta-ANP, may particularly be secreted in clinically stable hemodialysis patients.
本研究旨在阐明稳定血液透析患者血浆中心房利钠肽（ANP）的分子形式是否与健康志愿者不同，以及血浆中的ANP分子形式在血液透析治疗过程中是否会发生变化。10名无心脏病临床症状的稳定血液透析患者使用中空纤维型透析器进行了4小时的治疗。透析前血浆ANP浓度为210±101.6 pg/ml（平均值±标准差），显著高于志愿者（59.2±37.2 pg/ml，n = 25）。透析后显著降至71.6±60.1 pg/ml。通过凝胶渗透色谱法和反相高效液相色谱法测量ANP的分子模式。凝胶渗透色谱法中与真实的α-、β-和γ-ANP共迁移的免疫反应性α-ANP峰被认为是α-、β-和γ-ANP。4名患者的血浆在透析前含有β-ANP峰，其中3名患者在透析后仍含有β-ANP峰。这些结果表明，与真实β-ANP共迁移且被认为是β-ANP的中分子量ANP可能在临床稳定的血液透析患者中特别分泌。
