Ogawa K, Smith A I, Hodsman G P, Jackson B, Woodcock E A, Johnston C I
Clin Exp Pharmacol Physiol. 1987 Feb;14(2):95-102. doi: 10.1111/j.1440-1681.1987.tb00962.x.
A specific and sensitive radioimmunoassay has been developed and used to measure circulating atrial natriuretic peptide (ANP) in normal man and in patients with chronic renal failure. Circulating ANP levels rose with head-down tilt and exercise, and were raised in patients with chronic renal failure in proportion to volume status. This suggests that ANP release is mediated via increased atrial stretch, although other release mechanisms cannot be excluded. Extracts of normal human plasma subjected to reverse phase HPLC showed one major peak of immunoreactivity co-migrating with alpha-human ANP. However, when plasma extracts from patients with renal failure were chromatographed on a similar system, a second later eluting peak of ANP immunoreactivity was observed. This may represent circulating ANP precursors or degradation molecules. Significant arteriovenous differences in plasma ANP concentration were observed in patients with chronic renal failure. Arterial and venous plasma ANP levels decreased slightly after haemodialysis. Plasma ANP concentrations were inversely correlated with haematocrit in these patients.
已开发出一种特异性和敏感性均高的放射免疫分析法，并用于测定正常人和慢性肾衰竭患者循环中的心房利钠肽（ANP）。循环中的ANP水平随头低位倾斜和运动而升高，且在慢性肾衰竭患者中，其水平与容量状态成比例升高。这表明ANP的释放是通过心房牵张增加介导的，尽管不能排除其他释放机制。对正常人血浆提取物进行反相高效液相色谱分析，显示一个主要的免疫反应峰与α-人ANP共迁移。然而，当对肾衰竭患者的血浆提取物在类似系统上进行色谱分析时，观察到第二个较晚洗脱的ANP免疫反应峰。这可能代表循环中的ANP前体或降解分子。在慢性肾衰竭患者中观察到血浆ANP浓度存在显著的动静脉差异。血液透析后动脉和静脉血浆ANP水平略有下降。在这些患者中，血浆ANP浓度与血细胞比容呈负相关。
