Purdie C A, Piris J, Bird C C, Wyllie A H
Department of Pathology, University Medical School, Edinburgh, U.K.
J Pathol. 1995 Mar;175(3):297-302. doi: 10.1002/path.1711750307.
A consecutive series of 87 colorectal tumours were studied for loss of a polymorphic probe on chromosome 17q and of the 64 informative cases, 13 (20 per cent) showed loss of heterozygosity (LOH). Examples of LOH were found in carcinomas of all stages and in a large non-invasive adenoma. There was no correlation between 17q LOH and patient age, sex, standard clinicopathological variables (differentiation and nature of tumour margin), DNA ploidy, or tumour site, nor was 17q LOH associated with 17p LOH defined at four loci adjacent to p53. However, comparison of Dukes' B and C carcinomas revealed that tumours which had metastasized to regional lymph nodes at the time of primary surgery were significantly more likely to have lost this 17q allele. Clinical follow-up of this cohort of patients showed no significant difference in survival between patients whose tumours had lost or retained 17q. Thus, we conclude that 17q allele loss is associated with lymph node metastasis in locally aggressive colorectal tumours but probably not with blood-borne metastasis.
对连续的87例结肠直肠肿瘤进行了研究，检测17号染色体q臂上一个多态性探针的缺失情况。在64例信息充分的病例中，13例（20%）显示杂合性缺失（LOH）。在所有分期的癌以及一个大的非侵袭性腺瘤中均发现了LOH的例子。17q LOH与患者年龄、性别、标准临床病理变量（肿瘤分化程度和边缘性质）、DNA倍体或肿瘤部位之间均无相关性，17q LOH也与p53相邻四个位点定义的17p LOH无关。然而，对Dukes' B期和C期癌的比较显示，在初次手术时已转移至区域淋巴结的肿瘤，丢失该17q等位基因的可能性显著更高。对该组患者的临床随访显示，肿瘤丢失或保留17q的患者在生存率上无显著差异。因此，我们得出结论，17q等位基因缺失与局部侵袭性结肠直肠肿瘤的淋巴结转移有关，但可能与血行转移无关。
