Takita K, Sato T, Miyagi M, Watatani M, Akiyama F, Sakamoto G, Kasumi F, Abe R, Nakamura Y
Department of Biochemistry, Cancer Institute, Tokyo, Japan.
Cancer Res. 1992 Jul 15;52(14):3914-7.
To examine the role of loss of heterozygosity (LOH) during tumor development and/or progression, we looked for correlations between metastasis of breast cancer to a regional lymph node(s) and LOH of chromosomal arms 11p, 13q, 16q, 17p, and 17q, where frequent losses in primary tumors have been detected. No correlation between lymph node metastasis and LOH of chromosomes 13q, 16q, or 17q was observed. However, tumors showing LOH of chromosomes 11p (chi 2 = 10.82, P less than 0.01) and 17p (chi 2 = 6.78, P less than 0.01) revealed a significantly higher incidence of metastasis to a regional lymph node(s) than tumors without LOH on these chromosomal arms. Furthermore, only four of 30 (13%) patients with tumors that retained both 11p and 17p had metastasis to a regional lymph node(s), compared with 24 of 32 (75%) patients with tumors that had lost both 11p and 17p. Analysis of LOH with markers on chromosomes 11p and 17p in a large number of tumors indicated that the peritelomeric region of each of these chromosomal arms contains a tumor suppressor gene that may be associated with tumor progression, particularly metastasis to a regional lymph node(s).
为了研究杂合性缺失（LOH）在肿瘤发生和/或进展过程中的作用，我们探寻了乳腺癌转移至区域淋巴结与染色体臂11p、13q、16q、17p和17q的LOH之间的相关性，这些染色体臂在原发性肿瘤中常有缺失。未观察到淋巴结转移与染色体13q、16q或17q的LOH之间存在相关性。然而，显示染色体11p（卡方=10.82，P<0.01）和17p（卡方=6.78，P<0.01）LOH的肿瘤，其转移至区域淋巴结的发生率显著高于这些染色体臂无LOH的肿瘤。此外，在保留11p和17p的30例患者中，只有4例（13%）发生了区域淋巴结转移，而在同时缺失11p和17p的32例患者中，有24例（75%）发生了区域淋巴结转移。对大量肿瘤中染色体11p和17p上的标记进行LOH分析表明，这些染色体臂的每个端粒周围区域都包含一个肿瘤抑制基因，该基因可能与肿瘤进展，特别是区域淋巴结转移有关。
