Stemmer A
Marine Biological Laboratory, Woods Hole, MA 02543, USA.
J Microsc. 1995 Apr;178(Pt 1):28-36. doi: 10.1111/j.1365-2818.1995.tb03577.x.
The design of a scanned-cantilever-type force microscope is presented which is fully integrated into an inverted high-resolution video-enhanced light microscope. This set-up allows us to acquire thin optical sections in differential interference contrast (DIC) or polarization while the force microscope is in place. Such a hybrid microscope provides a unique platform to study how cell surface properties determine, or are affected by, the three-dimensional dynamic organization inside the living cell. The hybrid microscope presented in this paper has proven reliable and versatile for biological applications. It is the only instrument that can image a specimen by force microscopy and high-power DIC without having either to translate the specimen or to remove the force microscope. Adaptation of the design features could greatly enhance the suitability of other force microscopes for biological work.
本文介绍了一种扫描悬臂式力显微镜的设计，该显微镜完全集成到倒置高分辨率视频增强光学显微镜中。这种设置使我们能够在力显微镜就位的情况下，以微分干涉对比（DIC）或偏振方式获取薄光学切片。这种混合显微镜为研究细胞表面特性如何决定或受活细胞内三维动态组织的影响提供了一个独特的平台。本文介绍的混合显微镜已被证明在生物应用中可靠且通用。它是唯一一种能够通过力显微镜和高倍DIC对样本进行成像的仪器，而无需平移样本或移除力显微镜。对设计特点的调整可以大大提高其他力显微镜在生物工作中的适用性。
