Henderson E, Sakaguchi D S
Department of Zoology and Genetics, Iowa State University, Ames 50011, USA.
Neuroimage. 1993 Sep;1(2):145-50. doi: 10.1006/nimg.1993.1007.
A prototype combined optical fluorescence/atomic force microscope (OFAFM) designed for use in neurobiology and related disciplines has been constructed and used to study filamentous actin (F-actin) and other cellular structures in fixed Xenopus retinal glial cells (XR1 glial cell line). F-actin was readily observed by both fluorescence and AFM. AFM images of nuclei and other cellular structures were also obtained. The OFAFM consists of an AFM with an interferometer detection mechanism mounted on an inverted optical microscope. Integration of optical and scanned probe imaging methods provides a unique and useful approach to studying glial (and other) cell structure and function.
已构建了一种为神经生物学及相关学科设计的原型组合光学荧光/原子力显微镜（OFAFM），并用于研究固定的非洲爪蟾视网膜神经胶质细胞（XR1神经胶质细胞系）中的丝状肌动蛋白（F-肌动蛋白）和其他细胞结构。通过荧光和原子力显微镜都能轻松观察到F-肌动蛋白。还获得了细胞核和其他细胞结构的原子力显微镜图像。该OFAFM由一台安装在倒置光学显微镜上的具有干涉仪检测机制的原子力显微镜组成。光学成像方法与扫描探针成像方法的结合为研究神经胶质（及其他）细胞的结构和功能提供了一种独特且有用的方法。
