Nagase I, Sasaki N, Tsukazaki K, Yoshida T, Morimatsu M, Saito M
Department of Biochemistry, Faculty of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Jpn J Vet Res. 1994 Dec;42(3-4):137-45.
When mammals are exposed to a cold environment for a long time, the capacity of nonshivering thermogenesis by brown adipose tissue (BAT) increases in association with the increased expression of some specific proteins and tissue hyperplasia, which are totally dependent on sympathetic innervation to this tissue. To clarify roles of the beta-adrenergic mechanism in BAT hyperplasia, the effects of chronic administration of various beta-adrenergic agonists on BAT were examined in rats, especially focusing on some agonists to the beta 3-adrenoceptor which is present specifically in adipocytes. Chronic administration of noradrenaline or isoproterenol for 7-10 days produced a marked increase in the tissue contents of DNA, total protein, mitochondrial uncoupling protein, and insulin-regulatable glucose transporter protein. The trophic effects of noradrenaline and isoproterenol were mimicked by chronic administration of beta 3-adrenergic agonists, such as CL316,243, BRL 26830A, and ICI D7114. These results suggest that the beta 3-adrenoceptor plays important roles for hyperplasia of BAT, and thereby increasing in the capacity of thermogenesis.
当哺乳动物长时间暴露于寒冷环境时，棕色脂肪组织（BAT）的非颤抖性产热能力会随着一些特定蛋白质表达的增加和组织增生而增强，而这完全依赖于该组织的交感神经支配。为了阐明β-肾上腺素能机制在BAT增生中的作用，研究人员在大鼠中检测了长期给予各种β-肾上腺素能激动剂对BAT的影响，特别关注了脂肪细胞中特异性存在的β3-肾上腺素能受体的一些激动剂。连续7至10天给予去甲肾上腺素或异丙肾上腺素会使DNA、总蛋白、线粒体解偶联蛋白和胰岛素可调节的葡萄糖转运蛋白的组织含量显著增加。长期给予β3-肾上腺素能激动剂，如CL316,243、BRL 26830A和ICI D7114，可模拟去甲肾上腺素和异丙肾上腺素的营养作用。这些结果表明，β3-肾上腺素能受体对BAT的增生起着重要作用，从而增加产热能力。
