Wang H, Regunathan S, Youngson C, Bramwell S, Reis D J
Department of Neurology and Neuroscience, Cornell University Medical College, New York, NY 10021, USA.
Neurosci Lett. 1995 Jan 2;183(1-2):17-21. doi: 10.1016/0304-3940(94)11104-q.
Agmatine, a newly identified amine in mammalian brain, is an endogenous ligand for imidazoline and alpha 2-adrenergic receptors. We sought to develop a polyclonal antibody to agmatine suitable for immunocytochemistry. Agmatine was conjugated to keyhole limpet hemocyanin and injected into rabbits. The polyclonal antiserum so generated dose-dependently recognized the agmatine conjugate but not carrier protein by dot blot. Its reaction with the conjugate was selectively antagonized by agmatine but not related compounds. The antiserum, but not pre-immune or pre-adsorbed antiserum, selectively stained cultured adrenal chromaffin cells. Our results indicate that agmatine immunoreactivity is contained in a sub-population of adrenal chromaffin cells and, thus, these antibodies are useful for immunocytochemical localization of the amine in mammalian tissues.
胍丁胺是哺乳动物脑中新发现的一种胺类物质，是咪唑啉和α2 - 肾上腺素能受体的内源性配体。我们试图开发一种适用于免疫细胞化学的胍丁胺多克隆抗体。将胍丁胺与钥孔血蓝蛋白偶联后注射到兔子体内。如此产生的多克隆抗血清通过斑点印迹法呈剂量依赖性地识别胍丁胺偶联物，而不识别载体蛋白。其与偶联物的反应被胍丁胺选择性拮抗，而相关化合物则无此作用。该抗血清而非免疫前或吸附前的抗血清能选择性地对培养的肾上腺嗜铬细胞进行染色。我们的结果表明，肾上腺嗜铬细胞亚群中存在胍丁胺免疫反应性，因此，这些抗体可用于该胺类物质在哺乳动物组织中的免疫细胞化学定位。
