Vitolo D, Palmieri M B, Marzullo A, Ruco L P, Baroni C D
Department of Experimental Medicine, University La Sapienza, Rome, Italy.
Pathol Res Pract. 1994 Nov;190(11):999-1004. doi: 10.1016/S0344-0338(11)80893-9.
Lymphocytic infiltrates are usually present in chronic lymphocytic thyroiditis, thyroid papillary carcinoma and parotid adenolymphoma. We selected these conditions to investigate the mechanisms of recruitment and organization of lymphocytic infiltrates in extranodal tissues. MoAbs in immunoperoxidase were used to identify the expression of ICAM-1 and VCAM-1 on endothelial cells (EC), and of their ligands LFA-1 and VLA-4 on lymphocytes and accessory cells. VCAM-1 positive EC were rarely observed in thyroids devoid of lymphocyte infiltration. Conversely, EC in chronic lymphocytic thyroiditis and in papillary carcinoma showed positive immunostaining for VCAM-1 and ICAM-1. These findings were associated with the presence of lymphocytes positive for the ligands VLA-4 and LFA-1. The upregulated expression of VCAM-1 on perifollicular capillaries was co-distributed with an accumulation of VLA-4 positive lymphocytes. In adenolymphoma, all EC were ICAM-1 positive, whereas the majority of vessels were VCAM-1 negative. Consequently the majority of lymphoid cells were LFA-1 positive and VLA-4 negative. We suggest that ICAM-1 and VCAM-1 expression on EC play a role in the recruitment of lymphocyte infiltration in chronic lymphocytic thyroiditis and papillary carcinoma. Furthermore, the upregulation of VCAM-1 and VLA-4 in thyroid reactive and neoplastic conditions may be linked to an immune response possibly related to thyroid tissue antigens.
淋巴细胞浸润通常存在于慢性淋巴细胞性甲状腺炎、甲状腺乳头状癌和腮腺腺淋巴瘤中。我们选择这些病症来研究结外组织中淋巴细胞浸润的募集和组织机制。采用免疫过氧化物酶中的单克隆抗体来鉴定内皮细胞（EC）上细胞间黏附分子-1（ICAM-1）和血管细胞黏附分子-1（VCAM-1）的表达，以及淋巴细胞和辅助细胞上它们的配体淋巴细胞功能相关抗原-1（LFA-1）和极晚期抗原-4（VLA-4）的表达。在没有淋巴细胞浸润的甲状腺中很少观察到VCAM-1阳性内皮细胞。相反，慢性淋巴细胞性甲状腺炎和乳头状癌中的内皮细胞对VCAM-1和ICAM-1呈阳性免疫染色。这些发现与配体VLA-4和LFA-1阳性的淋巴细胞的存在有关。滤泡周围毛细血管上VCAM-1表达上调与VLA-4阳性淋巴细胞的聚集共同分布。在腺淋巴瘤中，所有内皮细胞ICAM-1均为阳性，而大多数血管VCAM-1为阴性。因此，大多数淋巴细胞LFA-1阳性而VLA-4阴性。我们认为内皮细胞上ICAM-1和VCAM-1的表达在慢性淋巴细胞性甲状腺炎和乳头状癌中淋巴细胞浸润的募集中起作用。此外，甲状腺反应性和肿瘤性病症中VCAM-1和VLA-4的上调可能与可能与甲状腺组织抗原相关的免疫反应有关。
