Lindahl T, Satoh M S, Dianov G
Imperial Cancer Research Fund, Clare Hall Laboratories, South Mimms, Hertfordshire, U.K.
Philos Trans R Soc Lond B Biol Sci. 1995 Jan 30;347(1319):57-62. doi: 10.1098/rstb.1995.0009.
Endogenous and environmental DNA-damaging agents often generate single-strand interruptions in DNA. The lesions trigger a complex set of cellular reactions. In most eukaryotic cells, cellular poly(ADP-ribose) formation is the most acute response to such damage. Recently, such events have been amenable to study with soluble cell-free extracts of human cells. These investigations clarify the modulating role on DNA repair by poly (ADP-ribose), and suggest that the primary function of this unusual polymer is to act as an antirecombinant agent. Similar biochemical studies of subsequent repair events have revealed a branched pathway for the ubiquitous DNA base excision-repair process. The alternative pathway provides the cell with back-up functions for individual steps in this essential form of DNA repair.
内源性和环境性DNA损伤剂常常会在DNA中产生单链断裂。这些损伤会引发一系列复杂的细胞反应。在大多数真核细胞中，细胞内聚（ADP - 核糖）的形成是对这种损伤最迅速的反应。最近，利用人类细胞的可溶性无细胞提取物已经能够对这类事件进行研究。这些研究阐明了聚（ADP - 核糖）对DNA修复的调节作用，并表明这种特殊聚合物的主要功能是作为一种抗重组剂。对后续修复事件的类似生化研究揭示了普遍存在的DNA碱基切除修复过程的一条分支途径。这条替代途径为细胞在这种基本的DNA修复形式中的各个步骤提供了备用功能。
