Kuznetsov A N, Livshits V A, Malenkov G G, Tenchov B G
Mol Biol (Mosk). 1976 Mar-Apr;10(2):386-94.
Some physico-chemical properties of lecithin lyposomes and the effects of pH, temperature and ionic strength changes are studied by ESR using hydrophobic and hydrophylic nitroxyl radicals as spin probes. The structure differences between outer and inner layers of bilayer liposomes are observed using auxiliary water soluble paramagnetic complex and attributed tentatively to the different curvature signs of both layers. Lower estimates of transversal diffusion rates of both probes throught the membrane are obtained using exchange broadening technique and water soluble reducing agent. The limiting step in this diffusion is revealed, caused by the transport of a charged group of the diffusing molecule through the hydrophobic membrane space.
使用疏水性和亲水性硝酰自由基作为自旋探针，通过电子自旋共振（ESR）研究了卵磷脂脂质体的一些物理化学性质以及pH值、温度和离子强度变化的影响。使用辅助水溶性顺磁配合物观察了双层脂质体外层和内层之间的结构差异，并初步归因于两层不同的曲率符号。使用交换展宽技术和水溶性还原剂获得了两种探针穿过膜的横向扩散速率的较低估计值。揭示了这种扩散中的限速步骤，其是由扩散分子的带电基团通过疏水膜空间的运输引起的。
