Danilov V S, Obraztsov V V, Rebrov V G, Orlov S N
Biofizika. 1977 Jan-Feb;22(1):54-7.
The nature of binding of ionic and neutral fluorescent probes in liposome structures of natural lecithin at various pH and ionic strength of the medium. Temperature relationship of fluorescence intensity of both probes presented in Arrhenius coordinates is shown to have bends at 32--42degrees C. A smaller bend in the temperature range of 20--40degrees C is fixed while studying the correlation time of the spin probe, line width of (CH2)n groups in the NMR-spectrum and light scattering at the angle of 90 degrees. Differences between the sensitivity of these procedures to the phase transition in the lipid structure and the method of measuring fluorescence probe intensity is discussed in terms of literature data.
在不同pH值和介质离子强度下，离子型和中性荧光探针在天然卵磷脂脂质体结构中的结合性质。以阿累尼乌斯坐标表示的两种探针荧光强度的温度关系表明，在32 - 42摄氏度时有弯曲。在研究自旋探针的相关时间、核磁共振谱中(CH2)n基团的线宽以及90度角的光散射时，在20 - 40摄氏度温度范围内发现了较小的弯曲。根据文献数据讨论了这些方法对脂质结构相变的敏感性差异以及荧光探针强度的测量方法。
