[薄层色谱后利用叶绿体进行除草剂检测]
[Use of chloroplasts for herbicide detection after thin layer chromatography].
作者信息
Trach V V, Shvartau v V, Dmitrenko N P, Chmil' V D
出版信息
Ukr Biokhim Zh (1978). 1994 Sep-Oct;66(5):100-4.
PMID:7747335
Abstract
The procedure of spreading of native pea (Pisum sativum L.) chloroplasts and 2,4-dichlorophenolindophenol on thin-layer chromatographical plates is used to detect herbicides. The sensitivity of photosystem 2 inhibitors detecting ureas and simm-triazines, is 10 ng. Dinitroaniline, thiocarbamate and chloroacetamide herbicides may be detected on 100 ng level. The method may by used for the screening of new physiologically active compounds for phototoxicity.
摘要
利用本地豌豆（豌豆）叶绿体和2,4-二氯酚靛酚在薄层色谱板上的铺展程序来检测除草剂。检测脲类和均三嗪类光系统2抑制剂的灵敏度为10纳克。二硝基苯胺、硫代氨基甲酸盐和氯乙酰胺类除草剂可在100纳克水平上被检测到。该方法可用于筛选具有光毒性的新型生理活性化合物。
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