Juhler R K, Sørensen S R, Larsen L
Department of Geochemistry, Geological Survey of Denmark and Greenland, GEUS, Thoravej 8, DK 2400, Copenhagen NV, Denmark.
Water Res. 2001 Apr;35(6):1371-8. doi: 10.1016/s0043-1354(00)00409-7.
High-performance liquid chromatography (HPLC) methods were developed for the optimised determination of five herbicide residues (dichlorprop, isoproturon, mecoprop, metsulfuron-methyl and 2,4,5-T) and major metabolites. These compounds represent important groups of herbicides and several residues have been found as contaminants in groundwater. The methods make it possible to study these herbicides and several transformation products through simultaneous detection and quantification. Culture media as well as cleaned up extracts from sediment and groundwater can be analysed. Using HPLC with UV detection the general limit of quantification was 1.8 ng injected corresponding to a detection limit of 1-2 micrograms/l when analysing a cleaned up extract from a 20 ml water sample. The method was verified by analysing herbicide residues in groundwater collected from a wetland area. Cleaning up 20 ml groundwater with a residue level of 25 micrograms/l the general recovery was within 58-82%.
开发了高效液相色谱（HPLC）方法，用于优化测定五种除草剂残留（二氯丙酸、异丙隆、甲草胺、甲磺隆和2,4,5-涕）及其主要代谢物。这些化合物代表了重要的除草剂类别，并且在地下水中发现了几种残留污染物。这些方法使得通过同时检测和定量来研究这些除草剂及其几种转化产物成为可能。可以分析培养基以及从沉积物和地下水中净化后的提取物。使用配备紫外检测的HPLC，在分析20毫升水样的净化提取物时，一般定量限为进样1.8纳克，对应检测限为1-2微克/升。通过分析从湿地地区采集的地下水中的除草剂残留对该方法进行了验证。对残留水平为25微克/升的20毫升地下水进行净化处理后，总体回收率在58-82%之间。
