Mavrutenkova T V, Shevtsova A I, Pis'menetskaia I Iu
Ukr Biokhim Zh (1978). 1994 Sep-Oct;66(5):110-4.
Spectra of lectin-binding proteins of the human trophoblast were studied by the method affinity blotting using a set of loctins conjugated with peroxidase. The set included lectins Lens culinaris (LcL), Canavalia ensiformis (Con A), Ricinus communis (RCA 1), Arachis hypogaea (PNA), Triticum vulgaris (WGA), Laburnum anagyroides (LAL). Presence of N- and O-glycans in the composition of proteins of plasmatic membranes of a trophoblast was shown, partial characteristics of their oligosugar chains being presented. Sialic acids were established to be absent in the composition of alkaline phosphatase of mature placenta.
采用一组与过氧化物酶偶联的凝集素,通过亲和印迹法研究了人滋养层细胞凝集素结合蛋白的光谱。该组凝集素包括菜豆凝集素(LcL)、刀豆球蛋白A(Con A)、蓖麻凝集素(RCA 1)、花生凝集素(PNA)、小麦胚凝集素(WGA)、金链花凝集素(LAL)。结果表明,滋养层细胞质膜蛋白成分中存在N-聚糖和O-聚糖,并呈现了其寡糖链的部分特征。研究还确定,成熟胎盘碱性磷酸酶的成分中不存在唾液酸。