Lu S, Santoro J C, Fuller D H, Haynes J R, Robinson H L
Department of Pathology, University of Massachusetts Medical Center, Worcester 01655, USA.
Virology. 1995 May 10;209(1):147-54. doi: 10.1006/viro.1995.1238.
Two DNA constructs encoding portions of the human immunodeficiency virus type-1 (HIV-1) genome have been used to raise antibody responses in BABL/c mice. One DNA (pNL4-3.env) expresses the natural form of the envelope glycoprotein (Env) of HIV-1-NL4-3 (NL4-3). The second (pNL4-3.dpol) produces noninfectious NL4-3 particles. These two DNAs (alone or in combination) raised only transient titers of anti-Env IgG. In the same group in which pNL4-3.dpol DNA raised only transient antibody responses to Env, this DNA raised persistent antibody responses to the p24 virion capsid protein (CA). Antibody responses to Env and CA also showed different abilities to be boosted. The final boosts of pNL4-3.dpol DNA increased titers of anti-CA antibody, but failed to boost the falling titers of anti-Env antibody. At peak titers of anti-Env activity, sera with relatively low ELISA titers of anti-Env IgG (end points of 1:6250) had good titers of neutralizing antibody (approximately 1:3800 for 50 TCID50 of NL4-3). At the end of the experiment (a time when anti-Env antibodies had fallen to near background levels), in vitro-restimulated splenocytes from both pNL4-3.env and pNL4-3.dpol DNA vaccine groups exhibited similar cytotoxic activity.
两种编码人类免疫缺陷病毒1型（HIV-1）基因组部分片段的DNA构建体已被用于在BABL/c小鼠中引发抗体反应。一种DNA（pNL4-3.env）表达HIV-1-NL4-3（NL4-3）包膜糖蛋白（Env）的天然形式。第二种（pNL4-3.dpol）产生无感染性的NL4-3颗粒。这两种DNA（单独或联合使用）仅引发了短暂的抗Env IgG滴度。在pNL4-3.dpol DNA仅引发对Env的短暂抗体反应的同一组中，该DNA引发了对p24病毒体衣壳蛋白（CA）的持续抗体反应。对Env和CA的抗体反应在增强能力上也表现出差异。pNL4-3.dpol DNA的最终增强提高了抗CA抗体的滴度，但未能提高逐渐下降的抗Env抗体滴度。在抗Env活性的峰值滴度时，抗Env IgG ELISA滴度相对较低（终点为1:6250）的血清具有良好的中和抗体滴度（对于50个TCID50的NL4-3，约为1:3800）。在实验结束时（抗Env抗体已降至接近背景水平的时间），来自pNL4-3.env和pNL4-3.dpol DNA疫苗组的体外再刺激脾细胞表现出相似的细胞毒性活性。
