Cartwright M, Mikheev A M, Heinrich G
Section of Biomolecular Medicine, University Hospital, Boston University Medical Center, MA 02118, USA.
Int J Dev Neurosci. 1994 Dec;12(8):685-93. doi: 10.1016/0736-5748(94)90048-5.
Brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF) and neurotrophin-3 (NT-3) are structurally related survival and differentiation factors for distinct sets of peripheral and central neurons. We previously reported that BDNF and NGF gene expression are differentially regulated in mouse L929 fibroblasts. Here we examine expression of these three neurotrophins in human fibroblasts. Northern blots detected BDNF and NT-3 mRNAs in fibroblasts derived from lung (WI-38), calvarium and foreskin. WI-38 cells and foreskin fibroblasts expressed 1.6 kb as well as 4 kb BDNF mRNAs whereas only the smaller BDNF mRNA was detected in calvarium fibroblasts. NGF mRNA was present in foreskin and calvarium but not lung fibroblasts. In WI-38 cells serum treatment increased levels of BDNF mRNA within 2 hr. Cycloheximide did not inhibit the increase. Treatment with 12-O-tetradecanoyl phorbol-13-acetate (TPA) transiently suppressed BDNF mRNA. Treatment with both serum and TPA first stimulated and then transiently suppressed BDNF mRNA. TPA and/or serum did not significantly affect BDNF mRNA in calvarium fibroblasts. These results show that human fibroblasts derived from different tissues express and regulate neurotrophin genes differentially.
脑源性神经营养因子（BDNF）、神经生长因子（NGF）和神经营养素-3（NT-3）在结构上相关，是不同组外周和中枢神经元的存活及分化因子。我们之前报道过，BDNF和NGF基因表达在小鼠L929成纤维细胞中受到不同调节。在此，我们检测这三种神经营养因子在人成纤维细胞中的表达情况。Northern印迹法在源自肺（WI-38）、颅骨和包皮的成纤维细胞中检测到BDNF和NT-3 mRNA。WI-38细胞和包皮成纤维细胞表达1.6 kb以及4 kb的BDNF mRNA，而在颅骨成纤维细胞中仅检测到较小的BDNF mRNA。NGF mRNA存在于包皮和颅骨成纤维细胞中，但不存在于肺成纤维细胞中。在WI-38细胞中，血清处理在2小时内增加了BDNF mRNA的水平。放线菌酮不抑制这种增加。用12-氧-十四烷酰佛波醇-13-乙酸酯（TPA）处理可短暂抑制BDNF mRNA。血清和TPA联合处理首先刺激然后短暂抑制BDNF mRNA。TPA和/或血清对颅骨成纤维细胞中的BDNF mRNA没有显著影响。这些结果表明，源自不同组织的人成纤维细胞表达并以不同方式调节神经营养因子基因。
