Higham S M, Edgar W M
Department of Clinical Dental Sciences, School of Dentistry, University of Liverpool, England.
Arch Oral Biol. 1995 Jan;40(1):55-9. doi: 10.1016/0003-9969(94)00140-7.
The effect of sucrose rinses supplemented with L-LDH and NAD was investigated. When LDH or NAD were added to the sucrose rinse, the pH fall was less marked than with rinsing with sucrose alone. Significant differences (p < 0.05) in mean minimum pH and cH areas were observed when sucrose rinses were supplemented with LDH, NAD or with a combination of LDH and NAD, when compared with sucrose alone. Plaque fluid concentrations of lactate and acetate significantly decreased with all rinses when compared with sucrose alone. Phosphate levels also decreased, although not significantly, when NAD was the sole supplement. No significant decreases were observed in succinate, formate or propionate concentrations. The results confirm the beneficial effect of LDH in reducing the accumulation of lactate in plaque following a sucrose rinse. The inclusion of NAD in the rinse demonstrated the role of extracellular hydrogen acceptors in the reaction. Further work is required to identify endogenous hydrogen receptors and to clarify the fate of pyruvate resulting from the oxidation of lactate before the potential role of LDH as a cariostatic agent can be fully understood.
研究了补充L - LDH和NAD的蔗糖漱口液的效果。当将LDH或NAD添加到蔗糖漱口液中时，pH值下降不如单独用蔗糖漱口时明显。与单独使用蔗糖漱口相比，当蔗糖漱口液补充LDH、NAD或LDH与NAD的组合时，平均最低pH值和cH面积存在显著差异（p < 0.05）。与单独使用蔗糖漱口相比，所有漱口液的菌斑液中乳酸和乙酸盐浓度均显著降低。当NAD作为唯一补充剂时，磷酸盐水平也有所下降，尽管不显著。琥珀酸盐、甲酸盐或丙酸盐浓度未观察到显著下降。结果证实了LDH在减少蔗糖漱口后菌斑中乳酸积累方面的有益作用。漱口液中加入NAD证明了细胞外氢受体在该反应中的作用。在充分理解LDH作为防龋剂的潜在作用之前，需要进一步开展工作以确定内源性氢受体并阐明乳酸氧化产生的丙酮酸的去向。
